Module1: Introduction to biocatalysis & biotransformation (1), Methods for new biocatalyst discovery (1), Enzyme assay (2), Purification and characterization of enzymes (2), Practical experimental methods for biotransformations (1).
Module 2 : Enzyme nomenclature (1), Immobilization of enzymes (1), Catalytic role of enzymes (4).
Module 3: Retrosynthetic biocatalysis (1); Enzymes in functional group transformation (16)
Module 4: White biotechnology (10)
An historical background to biocatalysis using enzymes and micro-organisms
Methods for new biocatalyst discovery
Origins of enzymes, microbial/plant/animal enzymes, screening methods (random screening and metagenomic approach)
Qualitative and quantitative assays, Highthroughput screening systems, Industrial perspective of enzyme assays, Enzyme finger printing
Enzyme purification and characterization
Different chromatographic methods
Experimental set up for an ideal biotransformation step
IUBMB system for enzyme classification and nomenclature
Immobilization of enzymes
Methods of immobilization (adsorption, covalent attachment, entrapment & Encapsulation)
Catalytic role of enzymes
Enzyme structure and function, co-factors and co-enzymes, Different mode of enzyme mechanisms (proximity effect, nucleophilic catalysis, GABC etc.)
Enzymes in functional group transformation
Functional group interconversion using enzymes (hydrolysis reaction, oxidation/reduction reactions, C-C bond formations), Retrosynthetic biocatalysis, Chemoenzymatic synthesis of natural products
Few industrial process using enzymes for production of drugs, fine chemicals and chiral intermediates.
General Organic Chemistry
Biotransformations in organic chemistry. Kurt Faber . Springer (ISBN : 3-540-66334-7).
Enzyme catalysis in organic synthesis (Vol I-III); Eds by K. Drauz and H. Waldmann. Willey-VCH (ISBN: 3-527-29949-1)
Hydrolases in organic synthesis (regio and stereoselective biotransformations). U. T. Bornscheuer and R. J. Kazlauskas. Willey-VCH. (ISBN: 3-527-30104-6).